Quantitative Estimation of Mangiferin and Molecular Docking Simulation of Salacia reticulata Formulation

Abstract

The phytochemical constituents present in herbal products need to be determined in their prescribed strength in order to ensure their efficacy and product quality in formulations. The marker-based standardization of herbal products is a well-accepted concept. In this study Mangiferin is used as marker to estimate the amount of Mangiferin present in the formulation Salacia Lin. Veggie capsules containing 400mg of Salacia reticulata extract to treat type 2 diabetes mellitus. The method was validated as per the international conference on harmonization (ICH) guidelines that is applicable in industry as well as in academia. The method was developed using reverse phase, Analytical column used for the separation of analytes, Phenomenex HPLC C18 (250 X 4.6 mm, 5 μm). The run time was of 7 min. The mobile phase used was acetonitrile and orthophosphoric acid (pH adjusted to 3.5) in the ratio 85:15 at a flow rate of 0.5ml/min, column temperature was maintained at 28°C and a detection wavelength of 257 nm using a photodiode array detector. The optimized method that was developed resulted in the elution of Mangiferin at 4.83min and % recovery was between 97.09 to 101.57. Molecular docking investigation was done using Schrodinger software. The binding affinity of phytoconstituents present in Salacia reticulata to intestinal enzymes alpha amylase was investigated to study their possible inhibitory mechanism. The physicochemical and drug-likeness properties of the phytoconstituents were evaluated. The phytoconstituent salacinol showed highest docking score (- 9.592) with alpha amylase (2QV4 obtained from protein data bank) and Mangiferin showed a score of -8.235 in comparison with standard acarbose ( -10.274). Studies showed phytoconstituents Mangiferin, Salacinol and Kotalanol can be potential inhibitors of 2QV4, and potent drug candidates for T2DM. However, further studies on these phytoconstituents should be carried out by wet lab experiments to prove their effectiveness.