Quantitative determination of trypsin inhibitor as a breeding marker in maize varieties with different resistance to fungal diseases

Galina Vladimirovna Shekhvatova,Viktor Vasilievich Ashin,Elena Fedorovna Sotchenko

doi:10.22363/2312-797x-2019-14-4-390-402

Abstract

Determination of plant resistance to fungal pathogens is an important breeding component. Thus, the study of protein profiles from corn kernels (13 genotypes) revealed a constitutively pronounced 14 kDa protein, trypsin inhibitor (TI), which is present at a relatively high level of concentration in seven Aspergíllus flavus - resistant maize lines, but at low concentrations or is absent in six sensitive lines. The 14 kDa trypsin inhibitor (TI) also showed antifungal activity against other mycotoxicogenic species. In this regard, the task was to determine the content of TI in varieties of maize with known properties, resistance, or sensitivity to such fungal pathogens of maize as head smut, common smut, and Fusarium stalk rot. According to the data obtained, the content of TI varies in different varieties and can vary by 4 times. However, in disease-resistant varieties its content is increased, which may be the primary marker of resistance of the variety to fungal pathogens.

Highlights

Plants are exposed to a large number of infectious pathogenic fungi
The samples of maize grains with known properties of resistance to fungal pathogens were provided from Russian Research Institute of Maize in Pyatigorsk
Peak areas obtained by trypsin-agarose chromatography were calculated by mathematical methods

Summary

Introduction

Plants are exposed to a large number of infectious pathogenic fungi. Common smut, head smut, stem rot, fusarium disease are the most common fungal pathogens in the Russian Federation. While in countries with a hot and arid climate, pathogens of the genus Aspergillus are most common, the effects of which are most fully studied. A study of protein profiles from maize grains (13 genotypes) revealed a constitutively expressed 14 kDa protein, a trypsin inhibitor (TI), which was present in relatively high concentrations in seven maize A. flavus resistant lines, but in low concen-. С. 390—402 trations or was absent altogether in six sensitive lines [1]. 14 kDa TI showed antifungal activity against other mycotoxicogenic species [2] С. 390—402 trations or was absent altogether in six sensitive lines [1]. 14 kDa TI showed antifungal activity against other mycotoxicogenic species [2]

Methods

Results

Conclusion