Abstract
Ractopamine (RAC) is banned for use in swine to improve carcass leanness in the vast majority of countries and areas because its residue may pose health risks. This study investigated the feasibility of applying Fourier transform infrared (FT-IR) spectroscopy ranging from 4000 to 400 cm−1 for analysis of RAC concentration in swine urine. RAC of 0–0.20 μg/mL was added to urine samples collected from 15 swines to prepare a total of 110 samples, which were dried at 40 °C for 48 h before spectra acquisition. By using the whole spectra pretreated by Savitzky-Golay smoothing (SGS)-spectral intensity normalization (SIN)-second derivative transformation (SDT), an optimal partial least squares regression (PLSR) model was obtained with a determination coefficient (R2) of 0.997 and a ratio of performance to deviation (RPD) of 18.756 for determining the RAC. Excitedly, 25 important wavenumbers could also be used to build a better PLSR model with R2 of 0.924 and RPD of 3.68. Compounds in swine urine could cause the shifts of some vibration peaks of RAC such as NH and OH via intermolecular interactions. The satisfactory results indicated that FT-IR spectroscopy combined with sample drying and spectra pretreatment has a great potential for rapid analysis of ractopamine in swine urine.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
