Quantitative Determination of Marker Compounds and Fingerprint Analysis of the Seeds of Vernonia anthelmintica.

Zulipiya Maimaiti,Ablajan Turak,Geyu Liu,Qing Ling Ma,Haji Akbar Aisa,Samuel Carda Broch

doi:10.1155/2020/8859425

Abstract

In traditional Chinese medicine, the seeds of Vernonia anthelmintica (L.) Willd. have been widely used for treatment of cough, skin diseases, diarrhea, fever, schistosomiasis, amoebic dysentery, and gastrointestinal problems, especially in the treatment of vitiligo for thousands of years in China. In this study, an effective, reliable, and accurate high-performance liquid chromatography diode array detector (HPLC-DAD) method was developed for quantitative analysis of 3 marker bioactive compounds and chemical fingerprint of the seeds of V. anthelmintica. Data corresponding to common peak areas and HPLC chromatographic fingerprints were analyzed by exploratory hierarchical cluster analysis (HCA) and principal component analysis (PCA) to extract information of the most significant variables contributing to characterization and classification of the analyzed samples. Based on variety and origin, the high-performance thin layer chromatography (HPTLC) method validated the chemical fingerprint results used to screen the in vitro antioxidant activity of V. anthelmintica. The results show that the developed method has potential application values for the quality consistency evaluation and identification of similar instant V. anthelmintica samples. When considered collectively, this research results provide a scientific basis for the improvement of standardization and specification of V. anthelmintica medicinal materials and provide a pathway for the development and utilization of references for the identification of V. anthelmintica herbs.

Highlights

Adequate and accepted research methodology for evaluating traditional Chinese medicine (TCM) has attracted much attention in recent years [1, 2]
In the previous studies about this plant, we found that three dicaffeoyl quinic acids (CQAs) play an important role in the treatment of vitiligo [23, 24], the 3 CQAs are an isomer and there is the possibility of interchanging each other, and they are high in V. anthelmintica. erefore, the quantitative determination of the 3 CQAs as marker compounds was studied in this article, and chromatographic fingerprinting was established by combination with a basic conventional analytical, highly precise, accurate, and novel pattern recognition method (HPLC-DAD) for quality control of V. anthelmintica herbs from different countries of origin
Validation of the HPLC Method of Quantitative Analysis, Calibration Curves, and Limits of Detection. e quantitative determination of the three marker compounds 3,4-CQA, 3,5-CQA, and 4,5-CQA in 26 batches of V. anthelmintica seeds was carried out using a HPLC-DAD, ensuring that the fingerprints are representative and authentic. e use of the optimal linear gradient methods resulted in a good separation of the three analytes and an excellent fingerprint chromatogram with a total analysis time below 40 min for separation of the three marker compounds (Figure 1) under the best detection wavelength conditions with 330 nm

Summary

Introduction

Adequate and accepted research methodology for evaluating traditional Chinese medicine (TCM) has attracted much attention in recent years [1, 2]. V. anthelmintica is an annual herbaceous species of the Asteraceae family, which is widely cultivated in high-altitude areas of southern Xinjiang and small regions in Pakistan and India [6]. It has long been used as a valuable traditional medicine in China for the treatment of cough, skin diseases, diarrhea [7], fever [8], schistosomiasis, amoebic dysentery, and gastrointestinal problems [9]. Many research studies have reported that V. anthelmintica contains many kinds of bioactive components such as sesquiterpenoids, flavonoids

Methods

Results

Conclusion