An HPLC-DAD Method to Quantify Flavonoids in Sonchus arvensis and Able to Classify the Plant Parts and Their Geographical Area through Principal Component Analysis

Abstract

A simple and efficient method has been developed for the simultaneous determination of eight flavonoids (orientin, hyperoside, rutin, myricetin, luteolin, quercetin, kaempferol, and apigenin) in Sonchus arvensis by high-performance liquid chromatography diode array detector (HPLC-DAD). This method was utilized to differentiate S. arvensis samples based on the plant parts (leaves, stems, and roots) and the plant’s geographical origin. The chromatographic separation was carried out on a reverse-phase C18 column by eluting at a flow rate of 1 mL/min using a gradient with methanol and 0.2% aqueous formic acid. In the optimum conditions, the developed method’s system suitability has met the criteria of good separation. The calibration curve shows a linear relationship between the peak area and analyte concentration with a correlation coefficient (r2) > 0.9990. The ranges for the analytes’ limits of detection and quantitation were 0.006–0.015 and 0.020–0.052 µg/mL, respectively. Intra-day and inter-day precision expressed in terms of RSD values were <2%, and the accuracy range based on recovery was 97–105%. The stability of all analytes within 48 h was about 2%. By combining HPLC-DAD fingerprint analysis with chemometrics, the developed method can classify S. arvensis samples based on the plant parts and geographical origin.