Abstract
Lycorine, a widespread alkaloid in Amaryllidaceae species, has been proven to have antiviral, cytotoxic and antimalarial activities [1–3]. A reversed-phase high-performance liquid chromatographic method has been developed and validated for the determination of lycorine in Amaryllidaceae plants. A simple method for the extraction of lycorine in low-mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut®) [4]. The chromatographic separation was performed using an isocratic system with a mobile phase trifluoroacetic acid-water-acetonitrile (0.01:90:10) and diode array detector [5]. The linearity of the method was studied by injecting five known concentrations of the standard in the range of 62.5–500µgmL-1. The calibration curve was determined as Y=23.9788285 X + 62.391901. Validation procedures showed that the method was specific, accurate and precise.
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