QUANTITATIVE DETERMINATION OF GALANTHAMINE AND LYCORINE IN GALANTHUS FOSTERI

Abstract

s. PHYTOPHARM 2012 M 66 Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] ТОМ 10/2012/2 QUANTITATIVE DETERMINATION OF GALANTHAMINE AND LYCORINE IN GALANTHUS FOSTERI © Kaya G. I., Emir A, Sarikaya B., Onur M. A., Unver Somer N. Department of Pharmacognosy, Faculty of Pharmacy, Ege University, Bornova Izmir 35100, Turkey Among the 14 taxa and one hybrid of the genus Galanthus growing in Turkey, Galanthus fosteri baker is distributed mainly in south-and north-central Turkey (1, 2). Galanthamine, the most important alkaloid found in Amaryllidaceae species, is used for the treatmant of Alzheimer’s disease (3). lycorine, a widespread alkaloid found in Amaryllidaceae plants has important biological activities (4). Therefore it is important to quantify these alkaloids in Amaryllidaceae species. in the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine and galanthamine in G. fosteri. The chromatographic separation was performed using an isocratic system with a mobil phase trifluoroacetic acid-water-acetonitrile (0.01: 92.5: 7.5) and diode array dedector (5). The linearity of the method was studied by injecting five known concentrations of lycorine in the range of 2.5–40 μg/ml and five known concentrations of galanthamine in the range of 1–16 μg/ml. The calibration curves for lycorine and galanthamine were determined as y = 15.6024x+0.4459 and y = 10.8302x-1.9292, respectively. The contents of galanthamine in the aerial parts was found to be 0.003 % whereas the amount of the lycorine was found as 0.010 %. The contents of galanthamine and lycorine in the bulbs of G. fosteri were found to be 0.012 % and 0.035 %, respectively.