Abstract
Gallotannins (tetra-undecagalloylglucose) in Paeoniae Radix were separated according to the degree of galloylation (number of galloyl groups per glucose) by normal phase high performance liquid chromatography and quantitiatively determined. Total gallotannin contents of Paeoniae Radix produced in Hokkaido, Nara prefecture and China were shown to be in the range of 0.52-1.16%, and there was no difference among three groups of samples. The average degree of galloylation was calculated from the contents of tetra-undecagalloylglucose, and those of Paeoneae Radix produced in Hokkaido (5.88-7.36) appeared to be higher than those in Nara prefecture (5.30-5.72) and China (5.10-5.75). The total gallotannin contents and the average degree of galloylation varied according to the preparation methods of Paeoniae Radix, and the total gallotannin contents in the aerial parts of peony were found to be 5-15 times higher than that of root on fresh weight basis. In the decoction of two Kanpo prescriptions, Shakuyaku-kanzo-to ( ?? ?? ?? ?? ?? ) and Keishi-ka-shakuyaku-to ( ?? ?? ?? ?? ?? ?? ), only tetra and pentagalloylglucoses were detected as gallotannin.
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Schedule a callMore From: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
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