Abstract
A homogeneous high-molecular-weight glycoprotein possessing tissue-specific, but not species-specific antimitotic activity relative to epithelium of dermal type (epidermal G2-chalone) was isolated electrophoretically and immunochemically from rat skin. A method of quantitative immunodiffusion determination of this substance by means of monospecific antiserum in certain tissues is described. Clear correlation between the mitotic index and concentration of G2-chalone in the mucous membrane at various stages of the estrous cycle was demonstrated in the vaginal epithelium.
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