Abstract
In this study, two different spectrophotometric methods (Vierordt and Absorbance ratio method) were applied for Cocaine HCl - Procaine HCl and Cocaine HCl-Caffeine in synthetic binary mixtures. The result of the spectrophotometric methods were then compared to the results obtained from an HPLC method. In the Vierordt method; A1 1 values (% 1.1 cm) were determined at 232, 221 and 272 nm in methanol for Cocaine HCl (I), Procaine HCl (II) and Caffeine (III), respectively. The ratio of A1 1 values and total absorbance values were applied to the equation which was developed by Vierordt. In the absorbance ratio method; 224 and 247 nm were chosen as isobestic points and the total absorbances were measured at 232, 296 and 272 nm in methanol. Absorbance ratios of 232/247 and 296/247 were used in the regression equation for I-II, also absorbance ratios of 231/224 and 272/224 were used in the regression equation for I-III synthetic binary mixtures. In the HPLC method, μ Bondopack C18 10 mμ column and methanol-water-phosphoric acid 1%-hexylamine (75:175:250:3,5) were used as mobile phase and antipyrine was chosen as internal standard for quantitative determination. The linear concentration areas were found in a range of 2–16 μg/mL in both spectrophotometric methods. The linear concentration interval was calculated between 2–25 μg/mL in the HPLC method. RSD values were found to be 0.83- 1.19- 1.33% for I, 1.05- 1.6- 0.94 % for II, 1.53-1.49–0.88% for III in Vierordt, Absorbance ratio and HPLC methods, respectively.
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