Quantitative detection of CC chemokine ligand 5 and matrix metalloproteinase-9 by QDs-based molecular prediction of breast cancer by

Abstract

Objective To explore the values for BC prediction in combination with CC chemokine ligand 5 (CCL5) and matrix metalloproteinasec (MMP)-9.Methods The quantitative and accurate determination of CCL5 and MMP-9 of 214 invasive BC tissue were acquired by QD-based immunohistochemistry (QD-IHC) and Wuda imaging analysis system.The relationships of quantitative CCL5 and MMP-9 determination with 3-year disease free survival (3-DFS) were investigated.Results CCL5 QDS of lymph node (-) group and lymph node (±) group was 36.952 ± 3.070 and 74.822 ± 3.582.CCL5 QDS of Lunminal A,Lunminal B,human epidermal growth factor receptor (HER-2) over-expression and triplenegative was 39.134 ±3.709,70.911 ±4.984,59.260 ±5.785,61.316 ±9.212.CCL5 QDS was correlated with lymph node metastasis and molecular sub-type (P ＜ 0.01).MMP-9 QDS of lymph node (-) group and lymph node (±) group was 18.912 ± 1.356,39.459 ±2.083.MMP-9 QDS was correlated with lymph node metastasis (P ＜ 0.01).There was a negative relationship between the expression of CCL5 (r =-0.329,P ＜ 0.01) and MMP-9 (r =-0.346,P ＜ 0.01) with 3-DFS.On multivariate analysis MMP-9 was indeed an independent predictor of 3-DFS (P ＜ 0.01,area under curve (AUC) [95％ confidence interval (CI):3.489 (1.736-7.011)].Conclusion QD-IHC nanotechnology can assess CCL5 and MMP-9 expression in BC accurately and quantitatively,reveal BC heterogeneity. Key words: Breast cancer; Quantum dots bioprobes; Chemokine ligand 5; Matrix metalloproteinasec-9