Abstract
Glycerides are the main components of oils, and fats, used in formulated products in the food and cosmetic industry as well as in the pharmaceutical product industry. However, there is limited literature available on the analysis of the chemical composition of glycerides. The lack of a suitable analytical method for complete chemical profiling of glycerides is one of the bottlenecks in understanding and controlling the change in chemical composition during processing, formulation, and storage. Thus, the aim of the present study is to develop a calibration-free quantitative proton nuclear magnetic resonance (qHNMR) method for the simultaneous quantification of different components of glycerides. The qHNMR method was developed for the quantification of mono-, di-, and triglycerides; their positional isomers; free fatty acids; and glycerol content. The accuracy, precision, and robustness of the developed method were evaluated and were found suitable for the quantitative analysis of five batches of marketed excipient. The study demonstrates the potential of qHNMR method for the quantification of different components of glycerides in various marketed products. The method has the ability to identify the variability of glycerides among different batches and suppliers in terms of chemical composition and also to discern the changes during storage.
Highlights
Glycerides are the main components of oils, and fats, used in formulated products in the food and cosmetic industry as well as in the pharmaceutical product industry
On the basis of the position of ester linkage, each of contain mono, di, and triglycerides that are synthesized via mono- and diglycerides exists in two positional isomeric esterification of glycerol molecules and fatty acid chains
We developed and qualified a quantitative solution-state 1H NMR for the quantification of different possible components of the MDG such as mono, di, and triglycerides and positional isomers of C16 and/or C18 saturated fatty acids (1-MG, 2-MG, 1,3-DG, 1,2-DG, and TG), glycerol (G), and free fatty acids (FA) (Fig. 1)
Summary
Glycerides are the main components of oils, and fats, used in formulated products in the food and cosmetic industry as well as in the pharmaceutical product industry. The aim of the present study is to develop a calibration-free quantitative proton nuclear magnetic resonance (qHNMR) method for the simultaneous quantification of different components of glycerides. The qHNMR method was developed for the quantification of mono-, di-, and triglycerides; their positional isomers; free fatty acids; and glycerol content. The study demonstrates the potential of qHNMR method for the quantification of different components of glycerides in various marketed products. Previous studies have used qHNMR acids) are quantified using chromatography with diverse in characterizing and quantifying the different hydrolytic detectors These methodologies are time-consuming, which products of triglycerides during lipolysis [4,5]. Limited or no literature is available, demonstrating the effect of aging on the chemical composition of the MDGs (including isomers). In continuation of the present work, we aim to establish correlating the current findings with the solid-state transformation of the MDG as a result of aging
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