Abstract
A quantitative capillary electrophoresis–mass spectrometry method has been demonstrated for the determination of methylphenidate in human urine over a dynamic range of 640. The samples were prepared by liquid–liquid extraction using 4 ml of human urine. The extracts were analyzed using a Finnigan LCQ ion-trap mass spectrometer in a two-event, positive-ion full-scan MS and tandem MS selected reaction monitoring mode. The lower level of quantitation was determined to be 1.5 ng/ml methylphenidate in human urine. The intra-assay precision had a relative standard deviation less than 6.8%. The intra-assay accuracy was less than ±14.7% bias from the nominal concentration for the full-scan MS analysis, and less than ±26.5% bias for the tandem MS analysis. Six incurred human urine samples containing methylphenidate were analyzed and a simple pharmacokinetic curve is presented.
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Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
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