Quantitative assessment of microplastic contamination in muddy shores of Gulf of Khambhat, India

Abstract

Microplastics (MPs) have become a global concern due to their widespread distribution in marine ecosystems. The present study was aimed to assess MPs contamination in 21 muddy shores sites situated in the Gulf of Khambhat. From each site, five samples (1 kg each) were collected. In the laboratory, the replicates were homogenated, out of which a 100 g sample was used for analysis. The total number of MPs, shape, colour, size and polymer composition of MPs were assessed. The MPs abundance ranged from 0.32 ± 0.18 particles/g (Jampore) to 2.81 ± 0.50 particles/g (Uncha Kotda) among different study sites. Moreover, threads were recorded maximum followed by films, foams and fragments. In case of MPs colour, black and blue coloured MPs occurred dominantly, with sizes ranging from 1 mm to 5 mm. FTIR analysis identified seven different types of plastic polymers, out of which polypropylene was the dominant plastic polymer (32.46 %) followed by polyurethane (32.16 %), acrylonitrile butadiene styrene (14.93 %), polystyrene (9.62 %), polyethylene terephthalate (4.61 %), polyethylene (3.71 %) and polyvinyl chloride (2.51 %). Based on the results of the Contamination factor (CF) value, Alang, Mahua, Ghogha and Uncha Kotda were identified as very high contaminated sites (CF ≥ 6). Pollution Load Index (PLI) value of entire study area revealed the Gulf of Khambhat as a polluted area with MPs contamination (PLI > 1). While value of Hazardous Index (H) identified 12 study sites as class-V risk category (H value > 10,000). Moreover, Pollution Risk Index (PRI) value revealed fifteen sites as very high contaminated sites (PRI > 1200). Pollution indices can be useful in predicting the level of MPs contamination at the study site. Overall, the present study provides the information on MPs contamination in the coastal region of the Gulf of Khambhat that can be used as a baseline data for future studies on the ecotoxicity of MPs on marine biota.