Abstract
Stevia rebaudiana, a perennial herb in the family Asteraceae, is a popular natural sweetener with sensory and functional attributes that outperform those of conventional high-potency sweeteners. Stevia rebaudiana leaves contain sweet glycosides that have biological effects. The various glycosides included in extracts must be measured since, in addition to the major glycosides, they may also contribute to activity. The isocratic HPLC method was validated for the identification of dulcoside A, steviolbioside, rebaudioside C, and rebaudioside B. The development and validation of the HPTLC technique for the simultaneous measurement of three steviol glycosides in Stevia rebaudiana leaves-steviolbioside, stevioside, and rebaudioside-A-also employed the ICH (International Conferences on Harmonisation) guidelines.
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