Abstract
A simple and reliable method of ultra-performance liquid chromatography with photodiode array detector (UPLC-PDA) was developed to control the quality of Radix Isatidis (dried root of Isatis indigotica) for chemical fingerprint analysis and quantitative analysis of eight bioactive constituents, including R,S-goitrin, progoitrin, epiprogoitrin, gluconapin, adenosine, uridine, guanosine, and hypoxanthine. In quantitative analysis, the eight components showed good regression (R > 0.9997) within test ranges, and the recovery method ranged from 99.5% to 103.0%. The UPLC fingerprints of the Radix Isatidis samples were compared by performing chemometric procedures, including similarity analysis, hierarchical clustering analysis, and principal component analysis. The chemometric procedures classified Radix Isatidis and its finished products such that all samples could be successfully grouped according to crude herbs, prepared slices, and adulterant Baphicacanthis cusiae Rhizoma et Radix. The combination of quantitative and chromatographic fingerprint analysis can be used for the quality assessment of Radix Isatidis and its finished products.
Highlights
Radix Isatidis is the dried root of Isatis indigotica Fort
Radix Isatidis has become an important component in various traditional Chinese medicine preparations (TCMPs), of which Banlangen Granules (BLGG) is widely used for toxic-heat removal and as an anti-viral drug in clinical practice
This study aims to reveal the correlation and consistency of quality control in crude herbs, prepared slices, and Radix Isatidis preparations
Summary
Radix Isatidis is the dried root of Isatis indigotica Fort. (Fam. Cruciferae), which is known as Banlangen (BLG) or Bei-Banlangen. Baphicacanthis cusiae Rhizoma et Radix is the dried rhizome and root of Baphicacanthus cusia (Nees) Bremek. Radix Isatidis even though both have been officially listed in Chinese Pharmacopoeia Numerous gratifying successes of the Radix Isatidis anti-viral effect have been reported [12,13,14,15]. Radix Isatidis has become an important component in various traditional Chinese medicine preparations (TCMPs), of which Banlangen Granules (BLGG) is widely used for toxic-heat removal and as an anti-viral drug in clinical practice
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