Abstract
A method based on accelerated solvent extraction (ASE) coupled with gas chromatography tandem mass spectrometry (GC-MS/MS) was developed for the quantitative analysis of spectinomycin and lincomycin in poultry egg (whole egg, albumen and yolk) samples. In this work, the samples were extracted and purified using an ASE350 instrument and solid-phase extraction (SPE) cartridges, and the parameters of the ASE method were experimentally optimized. The appropriate SPE cartridges were selected, and the conditions for the derivatization reaction were optimized. After derivatization, the poultry egg (whole egg, albumen and yolk) samples were analyzed by GC-MS/MS. This study used blank poultry egg (whole egg, albumen and yolk) samples to evaluate the specificity, sensitivity, linearity, recovery and precision of the method. The linearity (5.6–2000 μg/kg for spectinomycin and 5.9–200 μg/kg for lincomycin), correlation coefficient (≥0.9991), recovery (80.0%–95.7%), precision (relative standard deviations, 1.0%–3.4%), limit of detection (2.3–4.3 μg/kg) and limit of quantification (5.6–9.5 μg/kg) of the method met the requirements for EU parameter verification. Compared with traditional liquid–liquid extraction methods, the proposed method is fast and consumes less reagents, and 24 samples can be processed at a time. Finally, the feasibility of the method was evaluated by testing real samples, and spectinomycin and lincomycin residues in poultry eggs were successfully detected.
Highlights
Spectinomycin and lincomycin are aminoglycoside and lincosamide antibiotics, respectively, and they have synergistic and complementary effects on each other’s antibacterial spectra and antibacterial mechanisms
Considering that consumers have separate uses for whole eggs, albumens and yolks in hen, duck and goose eggs, we studied the elimination of spectinomycin and lincomycin residues in whole eggs, albumens and yolks
Compared with the LLE, solid-phase extraction (SPE) and core-shell molecularly imprinted solid-phase extraction (CSMISPE) methods, the accelerated solvent extraction (ASE) method has the advantages of a short extraction time, lower consumption of organic reagents and batch sample processing
Summary
Spectinomycin and lincomycin are aminoglycoside and lincosamide antibiotics, respectively, and they have synergistic and complementary effects on each other’s antibacterial spectra and antibacterial mechanisms. 30S subunit of ribosomes, and its antibacterial mechanism mainly involves preventing the binding of messenger ribonucleic acid and ribosomes, thereby hindering the synthesis of proteins and resulting in bactericidal effects [1]. The antibacterial mechanism of lincomycin mainly consists of binding to the bacterial ribosomal 50S subunit, which inhibits peptide acyltransferase, hinders the synthesis of. Foods 2020, 9, 651 bacterial proteins and results in bactericidal effects [2]. Spectinomycin and lincomycin are usually used in combination to treat infections with. Gram-positive bacteria and Gram-negative bacteria and are widely used to treat piglet diarrhea and infection by Mycoplasma hyopneumoniae and Mycoplasma pneumoniae, which cause chronic respiratory diseases in chickens [3,4,5,6]. The MRLs in the United States are 100–4000 μg/kg for spectinomycin in chicken and cattle muscle and liver and 100–600 μg/kg for lincomycin in pig muscle and liver
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