Detection and determination of spectinomycin and lincomycin in poultry muscles and pork by ASE-SPE-GC–MS/MS

Abstract

An innovative study on the qualitative identification and quantitative determination of spectinomycin and lincomycin residues in poultry (chicken, duck, and goose) muscles and pork using gas chromatography-tandem mass spectrometry (GC–MS/MS) was conducted. The samples were extracted and separated with accelerated solvent extraction (ASE) and then purified and enriched by solid-phase extraction (SPE). Then, the obtained residues were derivatized with 200 μL bis(trimethylsilyl) trifluoroacetamide (BSTFA) for 60 min at 75 °C, and the derivative products were injected into a GC–MS/MS system. Under the optimized conditions, the limits of detection (LODs) and limits of quantification (LOQs) of spectinomycin and lincomycin in samples were 2.5–4.6 μg/kg and 5.7–7.6 μg/kg, respectively, the targets’ recoveries were 79.7 %–94.2 %, the determination coefficients were 0.9992−0.9998, and the relative standard deviations (RSDs) were 1.2 %–3.5 %. The method revealed good linearity, and the parameters met the European Union requirements for the detection of veterinary drug residues. Poultry muscles and pork were procured from local stores for actual sample testing, confirming the feasibility and practicability of this method. Therefore, this study established an ASE-SPE-GC–MS/MS method to detect spectinomycin and lincomycin residues in animal-derived food.