Quantitative analysis of metabolites in urine using a highly precise, compact near-infrared Raman spectrometer

Abstract

This paper demonstrates the potential of a newly devised near-infrared Raman system in quantitative analysis of metabolites in urine. By using the system, Raman spectra have been measured for urine to which glucose, acetone, urea, or creatine was added artificially, and for urine including the four components simultaneously. The concentrations of glucose, acetone, urea, and creatine in urine which includes one particular component artificially have been determined by the intensities of Raman bands at 1130, 790, 1013, and 692 cm−1, respectively. The correlation coefficient (R) between the concentration of glucose, acetone, urea, or creatine and the Raman intensity has been calculated to be 0.981, 0.992, 0.991, and 0.998, respectively. Their detection limits have been found to be 41 mg/dl, 40 mg/dl, 4.9 mg/ml, and 1.5 mg/ml, respectively. The concentrations of glucose, acetone, urea, and creatine in urine including the four components simultaneously have also been determined by the intensities of the four bands. Again, good correlations were obtained between the concentrations and the Raman intensities with the R values of 0.924 (glucose), 0.987 (acetone), 0.962 (urea), and 0.923 (creatine), respectively.