Abstract
A rapid capillary electrophoresis method based on online acid barrage stacking has been successfully established for analysis of trace amounts of genistein in human plasma. Genistein was analyzed within 8 min, with 200 mmol L−1 citric acid (pH 1.7) as acid barrage, and injection times of 180 and 30 s for sample and acid, respectively. Good linearity was obtained in the range 0.05–5 mg L−1 and the limit of detection was 0.03 mg L−1. Compared with normal sample injection, this method resulted in more than a fiftyfold improvement in detection sensitivity. The technique has potential for use in studies of genistein metabolism and of exposure levels in humans.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
