Abstract
The fatty acid methyl ester mixtures obtainable from the National Heart Institute, Bethesda, Md., U.S.A., as reference standards for gas-liquid chromatography (GLC) have been examined in a number of laboratories. The results of experience with the mixtures are summarized and detailed recommendations are given of their use in assessing the accuracy of GLC equipment and procedures. The effects of several features of instrument design and operating conditions are discussed, and the recognition and correction of commonly encountered defects are described.
Highlights
Introduction of SampleTwo methods are commonly used for the introduction of samples of fatty acid methyl esters to gas-liquid chromatography (GLC) columns
There is a wide choice of detection systems, of liquid phases, of derivatives, and of conditions of
Two methods are commonly used for the introduction of samples of fatty acid methyl esters to GLC columns
Summary
Institute to make available to scientists in the lipid field a series of reference mixtures of known composition and of high purity This suggestion, prompted by the advent of gas-liquid chromatography and its impact on studies of lipid metabolism, led to the institution of a Program for Lipid Standards under the guidance of a program officer and aided by an advisory committee. This group has met on numerous occasions in order to define objectives for reference fatty acids, to plan the procural of reference compounds, and to compare the results of tests made in their own laboratories with the reference mixtures which were developed from their discussions.
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