Quantitative Analysis of Cytokines in Diabetic Nephropathy

Srinubabu Gedela,Gr Sridhar,Inavolu Lakshmi Maheswari,T Prabhakar,Girijasankar Girijasankar,Allam Appa Rao,Venkata Phani Bhushan Guruju,Mary Sulakshana

doi:10.4172/jpb.1000079

Abstract

Diabetes mellitus is the most important public health problems due to its high prevalence and enormous social and economic consequences. Diabetic nephropathy is one of the chronic complications of diabetes mellitus. Inflammatory mediators are believed to play a vital role as predictors of low-grade systemic inflammation in diabetic nephropathy. Pro- and anti-inflammatory mediators relationship between ageing and low-grade systemic inflammation could be determined by measuring pro- and anti-inflammatory mediator’s presence in the circulation in subjects of different age groups. To examine the impact of inflammatory mediators on diabetic nephropathy, we quantified important inflamma- tory mediators such as CRP, IL-10, IL-6, MPO and TNF-α in diseased and controls using standard ELISA assay. Correlation between the multiplexed assays of ELISA was good for CRP, IL-6, IL-10, TNF-α and myeloperoxidase. Within and between run impression values for the multiplex method was < 15%. As an easier and cheaper test for assessment of diabetic nephropathy, we here with recommend further studies of CRP in diabetic patients.

Highlights

Sufficient blood was collected by venipuncture into an EDTA venipuncture tube and centrifuged for 15 minutes at 1000× g and 4oc within 10 minutes after the blood collection
The course of diabetic nephropathy is mainly characterized by changes of urinary albumin excretion and glomerular filtration rate
The present study includes 60 people, these people are divided into four groups, among them 15 normal people come under control group, 15 type 2 diabetic patients group I, 15 type 2 diabetic nephropathy patients having 3mg/dl creatinine as group III

Summary

Introduction

Sufficient blood was collected by venipuncture into an EDTA venipuncture tube and centrifuged for 15 minutes at 1000× g and 4oc within 10 minutes after the blood collection. The results indicate that the CRP levels are more for Group III patients than Group I and Group II patients since low-grade systemic inflammation of type II diabetes increases the CRP levels. This indicates that type 2 diabetic nephropathy patients having >3mg/dl creatinine have more levels of CRP than the type 2 diabetic patients.

Results

Conclusion