Abstract
Ceramide III was prepared by the cultivation of Saccharomyces cerevisiae. Ceramide III was partitioned from the cell extracts by solvent extraction and analyzed by normal phased high performance liquid chromatography (NP‐HPLC) using evaporative light scattering detector (ELSD) and mass spectrometer. The mobile phase was composed of chloroform and methanol. One linear gradient mode was applied to gain proper analysis of cell extracts. Quantitative analysis of ceramide was performed. Based on the analytical condition, the effect of cultivation temperature for the production of ceramide was investigated and the optimum cultivation temperature was found to be 30°C at pH 7. From the results of mass spectrometer analysis, C26 ceramide was the dominant cell culture. The fragment ions from the mass spectrum of ceramides III, which were of various molecular weights, from m/z = 237–677, were observed.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
