Abstract
Ophthalmic microbial eye membrane is a kind of membrane complex with highly complex structure, but it also has the therapeutic effect of bacteria that can produce microbial eye membrane. Nowadays, there is no effective method to analyze the microbial membrane. Therefore, a quantitative analysis method of ophthalmic microbial membrane function based on microbiological analysis is proposed. The biomass per unit area, substrate coverage and average thickness of the biofilm were quantitatively analyzed with Staphylococcus as material and microbiological analysis method. The structure indexes such as biomass, average thickness and average diffusion distance increased significantly, indicating the transformation process of microbial membrane from occurrence to maturity. Microbiological analysis method can effectively evaluate the occurrence, development and maturation of microbial membrane, and has potential value in studying the theoretical mechanism of microbial membrane formation.
Highlights
In the natural and artificial environment, microorganisms are easy to adsorb on the surface of the eyes, and form a microbial membrane by secreting extracellular polysaccharides, proteins and nucleic acids
Microbial membrane is a kind of membrane complex with coordination, functionality and high structure, which can protect microorganisms and provide a good living environment for microorganisms [1]
Studies have shown that more than 90% of the environmental microorganisms live in the biofilm, and 80% of the bacterial diseases are related to the biofilm
Summary
In the natural and artificial environment, microorganisms are easy to adsorb on the surface of the eyes, and form a microbial membrane by secreting extracellular polysaccharides, proteins and nucleic acids. 3.4 Liquid chromatography tandem mass spectrometry for rapid detection Methanol was selected as the extraction solvent, and HLB column solid phase extraction method was used to extract the sample solution and eluent of microbial membrane to eliminate the interference of matrix. HLB column solid phase extraction method was used to extract the sample solution and eluent of microbial membrane.
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