Deciphering mono/multivalent draw solute-induced microbial ecology and membrane fouling in anaerobic osmotic membrane bioreactor

Abstract

Anaerobic osmotic membrane bioreactor (AnOMBR) attracted attention due to high quality effluent production with low energy demand, and draw solute has significant effect on the system performance. However, the mutual relationship between draw solute-induced salinity accumulation and microbial community had many unknown questions to be solved. This study purpose was to construct two AnOMBR to compare the impact of draw solutes of NaCl and MgCl2 on the dynamic change of microbial ecology and membrane fouling. The result indicated that the draw solute of MgCl2 caused less salinity and more membrane biofouling than that of the draw solute NaCl. Multiple microbiological analysis methods were applied to discover keystone species related to the conductivity change and membrane fouling, especially for the MgCl2-AnOMBR system. It was found that draw solute NaCl could benefit the growth of Proteobacteria to become the most abundant phylum to affect the membrane fouling, while Mg2+ introduction could stimulate the growth of NS9, Hydrogenphilaceae and Pedosphaeraceae to potentially cause the biofouling. Furthermore, phylogenetic molecular ecological networks (pMENs) deeply analyzed the microbial structure difference under Na+ and Mg2+ introduction, and indicated that the family Lentimicrobiaceae and Candidatus_Kaiserbacteria were the keystone species in NaCl-AnOMBR, while two genus Anaerolinea and SWB02, and two families Saprospiraceae and NS9 were discovered to have key effect in MgCl2-AnOMBR due to their strong extracellular polymeric substances (EPS) production ability for survival of other microorganisms. This study was significant to give microbial targets under the impact of various draw solutes, as the reference for the engineers to further investigate how to improve the microbial structure to enhance AnOMBR performance and inhibit the membrane biofouling.