Abstract

Side-chain backbone interactions (or “effects”) between nearest neighbours may severely restrict the conformations accessible to a polypeptide chain and thus represent the first step in protein folding. We have quantified nearest-neighbour effects ( i to i + 1) in peptides through reversed-phase liquid chromatography (RP-HPLC) of model synthetic peptides, where l- and d-amino acids were substituted at the N-terminal end of the peptide sequence, adjacent to a l-Leu residue. These nearest-neighbour effects (expressed as the difference in retention times of l- and d-peptide diastereomers at pHs 2 and 7) were frequently dramatic, depending on the type of side-chain adjacent to the l-Leu residue, albeit such effects were independent of mobile phase conditions. No nearest-neighbour effects were observed when residue i is adjacent to a Gly residue. Calculation of minimum energy conformations of selected peptides supported the view that, whether a l- or d-amino acid is substituted adjacent to l-Leu, its orientation relative to this bulky Leu side-chain represents the most energetically favourable configuration. We believe that such energetically favourable, and different, configurations of l- and d-peptide diastereomers affect their respective interactions with a hydrophobic stationary phase, which are thus quantified by different RP-HPLC retention times. Side-chain hydrophilicity/hydrophobicity coefficients were generated in the presence of these nearest-neighbour effects and, despite the relative difference in such coefficients generated from peptides substituted with l- or d-amino acids, the relative difference in hydrophilicity/hydrophobicity between different amino acids in the l- or d-series is maintained. Overall, our results demonstrate that such nearest-neighbour effects can clearly restrict conformational space of an amino acid side-chain in a polypeptide chain.

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