Abstract
The electroimmunoassay for Clr‐Cls complexes was performed in two electrophoretic steps, the first in the presence of Ca2+ without antibody, and the second in the presence of EDTA with anti‐Clr in the gel. The results were reproducible and in agreement with semiquantitative estimates of Clr‐ClS complexes in normal and pathological sera on crossed immunoelectrophoresis.
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