Abstract
Abstract The amount of measles-specific IgG in sera, CSF, and brain extracts from patients with SSPE was determined by radial immunodiffusion method with the use of a number of rabbit antisera specific for human γ-chain, after each sample was abosrbed suitably with a lyophilized preparation of measles-precipitating antigen (MPA) made in Vero cells infected with Edmonston strain of measles virus. The measles-specific IgG contents of the sera ranged from approximately 10 to 20% and those of CSF and brain extracts from 25 to 75% of the total IgG, indicating a synthesis of measlesspecific IgG within the CNS. By employing the same method, a significant amount of measles-specific IgM was demonstrated in sera of three out of six SSPE patients. Sera, CSF, and brain extracts from controls when absorbed with MPA and quantitated by the same method did not show significant amounts of measles-specific IgG. When the agarose gel electrophoretic patterns of unabsorbed and MPA-absorbed SSPE brain extracts were compared, the absorbed extracts were found to lack homogeneous bands in a γ-globulin region in contrast to the presence of two to three homogeneous IgG bands seen in the unabsorbed brain extracts. On the other hand, the patterns of unabsorbed and absorbed SSPE sera did not show such a significant difference in γ-globulin region. These data strongly support the concept that measles virus is responsible for the formation of homogeneous bands within the CNS in SSPE.
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