Quantitation of arsenic species in urine for exposure assessment studies

Abstract

Accuracy in trace level clinical or biomedical analyses is of increasing importance as populationbased surveillance is more widely used to address issues of nutritional requirements, environmental exposures, and epidemiological outcomes. Among the uses of such surveillance, evaluation of community exposures from a local arsenic pollution source is one example. Urinary biological monitoring is a desirable tool used in conjunction with measurement of environmental levels of arsenic in order to permit assessment of total exposures and to provide a basis for consideration of the relative importance of different routes of exposure and of age-related behaviors in influencing exposure. Urinary arsenic concentrations are indicative of recent (previous 1-2 days) exposures to arsenic. When total urinary arsenic is measured, total arsenic intake is assessed. When exposures to inorganic arsenic (in the forms of oxides or salts of trivalent or pentavalent arsenic) are of interest, determination of urinary arsenic species comprising the major metabolites of inorganic arsenic exposures is most appropriate [1]. A recent two-year study of community exposures to environmental inorganic arsenic pollution utilized urinary arsenic speciation analysis to assess exposures. In these analyses, part-per-billion sensitivity was achieved with a mean precision of less than 12% coefficient of variation, and with excellent interlaboratory comparability.