Quantitation and test of enantiomeric purity of the l-ketohexoses by liquid chromatography with dual refractive index and laser-based chiroptical detection

Abstract

Experimental data for the synthetic methods of preparation of the l-ketohexoses have been lacking an analytical method for monitoring the chemical or enzymatic reactions described. Dual refractive index and laser-based chiroptical detection provides an ideal method for following the reactions, since the refractive index detector quantifies the amount of analyte, while the ratio of optical rotation to refractive index response allows the enantiomer mole fraction to be determined. Sulfonated polystyrenedivinylbenzene resin in the Ca form as the stationary phase with H 2O at 80 °C as the eluent gives base-line separation of sorbose, fructose, tagatose, and psicose. Dependent on the complexity of the reaction mixture, analysis times range from 20 to 60 min.