Quantifying the temporal expression of the Staphylococcus aureus collagen adhesin

Abstract

The initial event in the pathogeneis of most Staphylococcus aureus infections is attachment to biological substrates in vivo using specific protein–protein interactions. We previously quantified the temporal expression of the collagen adhesin (CNA) on S. aureus Phillips and demonstrated that dynamic adhesin capacity to collagen is strongly influenced by the adhesin density. However, most strains of S. aureus express an extensive amount of Protein A on the surface, presenting a complication when using immunofluorescence to quantify specific surface adhesins. In this study, an improved adhesin quantification method is presented that accommodates variable surface Protein A levels. This method was used to examine the temporal expression of CNA on six S. aureus strains. Collagen adhesin levels varied with growth phase and strain, ranging from 1096 to 8950 copies per cell. Five of the six strains exhibited a temporal expression pattern similar to that previously reported for S. aureus Phillips.