Quantification of Very Low Concentrations of Colloids with Light Scattering Applied to Micro(Nano)Plastics in Seawater

Abstract

The detection and quantification of micro(nano)plastics in the marine environment are essential requirements to understand the full impacts of plastic pollution on the ecosystem and human health. Here, static light scattering (SLS) and dynamic (DLS) light scattering techniques are assessed for their capacity to detect colloidal particles with diameters between d = 0.1 and 0.8 µm at very low concentrations in seawater. The detection limit of the apparatus was determined using model monodisperse spherical polystyrene latex particles with diameters of 0.2 µm and 0.5 µm. It is shown that the concentration and size of colloids can be determined down to about 10−6 g/L. Light scattering measurements on seawater obtained from different locations in Western Europe show that colloidal particles were detected with DLS in seawater filtered through 0.8 µm pore size filters. The concentration of these particles was not higher than 1 µg/L, with an average diameter of about 0.6 µm. We stress that these particles are not necessarily plastic. No particles were detected after filtration through 0.45 µm pore size filters.