Quantification of Triterpene Saponins of Hedera Helix Spray-Dried Extract by HPLC

Abstract

Traditionally, ivy leaf (Hedera helix) has been used for a large number of ailments. In the 19th century, water-based extract of young leaves was used for respiratory diseases. The aim of the current study was to quantify the triterpene saponin named hederacoside C of ivy leaf spray-dried extract of Hedera helix using high-performance liquid chromatography (HPLC) method coupled with UV-visible detector. The solvent system consisted of solvent A (phosphoric Acid 85%: acetonitrile: water with the ratio of 2: 140: 860) and solvent B (phosphoric acid 85%: acetonitrile with the ratio of 2:998). A gradient elution was used for separation at 205 nm at a flow rate of 1.5 mL/min. The separation was performed using a C18 column at a temperature of 40°C. The method was validated as per ICH guidelines for precision, accuracy, recovery, ruggedness, and robustness. The method was found to be precise, accurate, robust, and reproducible according to the guidelines of United States Pharmacopeia 2014 and European Pharmacopeia 8.0. The content of triterpene saponins was found to be 17.6%. Despite many studies reported on the method development and quantification of compounds of Hedera helix, there is insufficient work reported on the spray-dried extracts of this plant. This study quantifies the hederacoside C from the spray-dried extract of the plant by developing an accurate, cheap, robust, and precise method. The proposed method can be of significant usage in the pharmaceutical industry.