Abstract
In the course of our ongoing work on the chemical characterization of Corsican olive oil, we have developed and validated a method for direct quantification of squalene using 13C Nuclear Magnetic Resonance (NMR) spectroscopy without saponification, extraction, or fractionation of the investigated samples. Good accuracy, linearity, and precision of the measurements have been observed. The experimental procedure was applied to the quantification of squalene in 24 olive oil samples from Corsica. Squalene accounted for 0.35–0.83% of the whole composition.
Highlights
IntroductionCDCl3 has been conserved as solvent and trioleine has been used as a model for olive oil; Longitudinal relaxation times have been measured for carbons of squalene by the inversion-recovery method
Squalene plays a major role in the reduction of cancer risks, with regard to cancer of the pancreas and colon in rodents [2,3,4]
Squalene increases the stability of various emulsions [5,6]
Summary
CDCl3 has been conserved as solvent and trioleine has been used as a model for olive oil; Longitudinal relaxation times have been measured for carbons of squalene by the inversion-recovery method. They ranged from 0.4 to 10.0 s, the highest values (4.1–10.0 s) being measured, as expected, for quaternary carbons (Table 1). Olive oil coming from two varieties of fruits contained 0.37–0.67% of squalene
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