Abstract
Objective: TLC densitometric method for quantification of gallic acid, quercetin and lupeol using HPTLC is developed. This is the first report of quantification of these three bioactive compounds viz. Gallic acid, quercetin and lupeol using HPTLC from this plant. Methodology: Quantification of gallic acid and quercetin was carried out from the methanolic extract using the solvent system of Toluene:Ethyl acetate:Formic acid (6:4:0.8 v/v/v). Lupeol was quantified from chloroform extract using the solvent system of Toluene:Ethyl acetate (7:3 v/v). Results: The Rf values of gallic acid, quercetin and lupeol are 0.22, 0.37 and 0.70 respectively. The linearity ranges for gallic acid (100 to 600 ng), quercetin (2000 to 7000 ng) and lupeol (100 to 1200 ng) with correlation coefficients (r-values) of 0.99968, 0.99708, and 0.99971 respectively. The amount of gallic acid, quercetin and lupeol was 124.31, 580.4, 24.89 μg/ml respectively. Conclusion: Quantification of gallic acid, quercetin and lupeol showed good resolution and separation from other constituents of extract. Its main advantages are its simplicity, accuracy, and selectivity. This method can also be used for the estimation of these compounds in other herbal preparations and may be useful for standardization purposes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Analytical & Bioanalytical Techniques
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
