Quantification of gallic acid in fruit and leaves of Careya arborea by High performance thin layer chromatography (HPTLC)

Abstract

Objective: The present study was aimed to quantification of Gallic acid in Careya arborea (Lecythidaceae). Method: High performance thin layer chromatography (HPTLC) quantification was carried out for various extract of leaf and fruits of C. arborea. The thin layer chromatography (TLC) plate was spotted with gallic acid and fruits and leaves extract of Careya arborea. The extract of fruit and leaves shows the Gallic acid spot at Rf: value 0.68 using mobile phase ethyl acetate: toluene: formic acid (8:2:0.3, v/v). So, the gallic acid was selected as marker. The gallic acid was quantified using HPTLC technique in CFE (C. arborea fruit ethyl acetate extract), CFA (C. arborea fruit alcoholic extract), CLE (C. arborea leave ethyl acetate extract) and CLA (C. arborea alcoholic extract of leaves) of Careya arborea. Result: The percentage (w/w) amount of gallic acid was found to 2.11%, 1.2 %, 0.54% and 0.48% in CFE, CFA, CLE and CLA of C. arborea respectively. Conclusion: This HPTLC quantification method useful for standardization of fruits and leaf Careya arborea.