Abstract
Evaluation of quantitative variation of secondary metabolites in Bergenia ciliata (Haw.) using high performance thin layer chromatography.
Highlights
Dear Editor: Therapeutically active metabolite contents in a medicinal plant vary in nature, which may impact on its therapeutic efficacy
We analyzed three accessions of Bergenia ciliata collected from different altitudes of Uttrakhand, India, to study the quantitative variation in bergenin, gallic acid, (+) catechin, and gallicin by high performance thin layer chromatography (HPTLC)
We found significant variations in the content of these compounds in the collected rhizomes of Bergenia ciliata ranging from 0.756¡0.3 to 0.85¡0.2 for bergenin, 0.145¡0.2 to 0.27¡0.2 for (+) catechin, 0.027¡0.2 to 0.166¡0.3 for gallic acid and 0.135¡0.3 to 0.186¡0.1 for gallicin
Summary
Dear Editor: Therapeutically active metabolite contents in a medicinal plant vary in nature, which may impact on its therapeutic efficacy. Its distribution over a wide range of altitudinal zones makes it a good candidate for studying variations in its metabolic profiles under different climatic conditions. Bergenin (C-glycoside of 4-O-methyl gallic acid) has been identified as a potent active secondary metabolite in Bergenia and other therapeutically active constituents including, among others, gallic acid (3,4,5 trihydroxybenzoic acid), (+) catechin, and gallicin (Fig. 1).
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