Quantification of Nitrite/Nitrate in Food Stuff Samples Using 2-Aminobenzoic Acid as a New Amine in Diazocoupling Reaction

Abstract

2-Aminobenzoic acid has been used as an amine in diazocoupling reaction to form an azo dye in the quantification of nitrite/nitrate at trace level. The formed azo dye has an absorption maximum at 550 nm in aqueous phase, and the resulted dye can be extracted into organic solvent to lower the detection limit. The method obeys Beer's law in the concentration range 0–10 µg of nitrite in 25 ml of aqueous solution with a molar absorptivity of 3.6 × 103 L mol−1 cm−1 and 0–2 µg of nitrite in 5 ml of organic phase. The detection limit of the dye has been found to be 0.056 μg ml−1. Nitrate is determined by reducing it to nitrite after passing through a copperized cadmium reductor column. The effect of interfering ions on the determination of nitrite/nitrate has been described. The developed method has been applied to determine the nitrite/nitrate trace level in vegetable, fruit juice, and milk powder samples.