Abstract
Objective: Osteoarthritis can be treated by taking oral supplements containing compounds that can nourish bones and joints such as hyaluronic acid,methylsulfonylmethane (MSM), chondroitin, glucosamine, and collagen. This study aimed to develop and validate tests for analyzing two compounds,namely, hyaluronic acid and MSM, simultaneously and to determine both their levels in a mixed sample.Methods: Hyaluronic acid derivatization was carried out using fluorenylmethyloxycarbonyl chloride and then analyzed by liquid chromatographywith fluorescence detection, while MSM was analyzed using gas chromatography. After the development of optimal conditions for each separation,system suitability tests were developed and calibration curves used for tests of accuracy and precision as well as for level determination. Hyaluronicwas detected at an excitation wavelength of 255 nm and emission wavelength of 330 nm. The mobile phase used was acetonitrile-acetate pH 4.2 (1: 4)with a flow rate of 1.0 mL/min.Results: The developed method was linear (r=0.9983) in the range of 5–50 ppm and the limits of detection (LOD) and quantitation (LOQ) were 3.55and 11.84 ppm, respectively. The initial column temperature for MSM analysis was 110°C and the mobile phase used was nitrogen gas at a flow rate of0.8 mL/min. The method was linear (r=0.9998) in the range of 4000–15,000 ppm and the LOD and LOQ were 332.90 and 1109.67 ppm, respectively.Conclusion: A simulated sample containing both compounds was assessed to contained 98.63% hyaluronic acid and 99.35% MSM.
Highlights
Food supplements are products that contain nutrients and excipients and are available as capsules, tablets, powders, or liquids; they are used in cases where dietary insufficiency of their ingredients can lead to ill health [1]
There are a number such supplement products sold to treat the symptoms of osteoarthritis and these usually contain glucosamine sulfate, chondroitin sulfate, MSM, Celadrin, nattokinase, hyaluronic acid, black catechu, skullcap, Boswellia serrata, and curcumin [3]
This study developed analytical methods for only hyaluronic acid and MSM because separation all these components require high-performance gradient liquid chromatography, while only isocratic high-performance liquid chromatography (HPLC) was available
Summary
Food supplements are products that contain nutrients and excipients and are available as capsules, tablets, powders, or liquids; they are used in cases where dietary insufficiency of their ingredients can lead to ill health [1]. Food supplements are only complementary, and not intended to be used as substitutes for a normal diet. These supplements usually contain chemical compounds that function as nutrients in the body and they can aid in supplying the specific nutritional needs of those with certain health conditions. Two compounds that are often used in supplements to prevent these conditions are hyaluronic acid and methylsulfonylmethane (MSM), both of which can nourish bones and joints [1]. This study developed analytical methods for only hyaluronic acid and MSM because separation all these components require high-performance gradient liquid chromatography, while only isocratic high-performance liquid chromatography (HPLC) was available
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