Abstract
Type 2 diabetes mellitus (T2DM), a worldwide disease that affects the quality of human life and social development. Glucose, fructose and 1,5-anhydroglucitol are closely related to diabetes mellitus. However, few methods have been reported to achieve these three carbohydrates in the blood simultaneously. In this study, a UPLC-MS/MS method allowing to quantify glucose, fructose, and 1,5-anhydroglucitol simultaneously in human plasma was developed. The analysis was performed by UPLC-MS/MS system with HILIC column. This new method provided satisfactory results in terms of calibration curves with good linearity (R2 > 0.99) over 3 order of magnitude range, precision (coefficient of variation of intra-day and inter-day: 0.72–10.23% and 2.21–13.8%), accuracy (results of intra-day and inter-day: 97–113%, 100–107%), matrix effects (87–109%), recovery (93–119%), carry-over (0.004–0.014%), as well as stability (0.04–6.9%) within the acceptance criteria. The reproducible, precise and accurate method with suitable dynamic ranges was successfully applied to the analysis of glucose, fructose and 1,5-anhydroglucitol in T2DM under different pathophysiological conditions.
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