Quantification of Acetaminophen, Caffeine and Ibuprofen in Solid Dosage Forms by UV Spectroscopy coupled with Multivariate analysis

Abstract

Quantification of acetaminophen, caffeine and ibuprofen in ternary mixtures was studied by using UV spectroscopy coupled with multivariate analysis [tri-linear regression-calibration (TLRC), classical least squares (CLS) and multi linear regression calibration (MLRC)]. To validate UV spectroscopic methods, the wavelength range of 215 – 290nm and a matrix (composed of 21 standard solutions containing acetaminophen 20-32.5mg/L, caffeine 1-3.5mg/L and ibuprofen 12-32mg/L) were selected. With the exception of TLRC, CLS and MLRS algorithms proved to be relevant for obtaining UV spectroscopic assay results and dissolution profile of acetaminophen, caffeine and ibuprofen in their combined capsules and tablets, with precision (RSD < 3%) and accuracy (98.3 – 101.9% recovery). It is also suggested that these UV spectroscopic methods could replace UHPLC analysis in the routine quality control of these compounds in their solid pharmaceutical dosage forms (p > 0.05).