Quantification chemometrically assisted of veterinary quinolones in edible animal tissues by excitation-emission fluorescence-kinetic data obtained through Fenton degradation

Abstract

Four quinolones (marbofloxacin, sarafloxacin, danofloxacin and enrofloxacin) were simultaneously quantified in edible animal tissues. A strategy based on third–order data, excitation–emission matrices as function of the time reaction during their Fenton degradation were employed to build a four–way calibration model. Unfolding partial least–squares (U–PLS) coupled to trilinearization residual (RTL) was the algorithm of choice to overcome both the high spectral overlapping among the analytes, and also the background signal present in the complex matrices. Bovine liver and porcine meat tissues were fortified with the analytes under study at different concentration levels, considering the maxima residual levels (MRLs) established by European Community. A recovery test was performed attaining recovery values ranging from 86 % to 123 % for all the analytes. In accordance with the principles of Green Analytical Chemistry, the proposed methodology enables the determination of the quinolones in complex matrices involving a simple extraction/clean–up procedure, a short time of analysis, and a no sophisticated instrumental. • A novel methodology to quantify quinolones in edible animal tissues is presented. • The method, environmentally friendly, involves a simple extraction/clean-up procedure. • Excitation–emission fluorescence matrices modulated by the kinetic of Fenton reaction are obtained. • Third-order data are obtained in a fast and simple way. • U–PLS coupled to RTL is employed to analyze the obtained four–way arrays.