Abstract
The quantities of ethanolamine glycerophosphatides (EGP), choline glycerophosphatides (CGP), and serine glycerophosphatides (SGP) were determined in the grey and white matter from three apparently normal adult human brains. In each locale the quantities decreased from EGP through CGP to SGP. The quantities of aldehydes in these lipids were also determined. In grey matter the aldehyde content (expressed as per cent of fatty acids plus aldehydes) of EGP, CGP, and SGP was 22, 0.3, and 0.2% respectively; while in white matter the proportions were 49, 0.8, and 13%, respectively. Palmitaldehyde, stearaldehyde, and olealdehyde made up 90% of the aldehydes found. White matter glycerophosphatides contained much more olealdehyde than those from grey matter. EGP and SGP contained much larger proportions of C20 and C22 polyenoic acids from grey matter than from white matter. CGP, on the other hand, had a similar fatty acid composition, comprised mainly of 16:0, 18:0, and 18:1 acids, in each locale. The differences in fatty acid composition of these three glycerophosphatides may be related to the higher myelin content of white matter.
Highlights
The quantities of aldehydes in these lipids were determined
The column chromatographic procedures used for isolation of ethanolamine, serine, and choline glycerophosphatides (EGP, Serine glycerophosphatides (SGP), and CGP)' have been outlined previously [3, 4].One scheme used for isolation of these lipids was as follows
The ethanolamine glycerophosphatide fraction isolated from adult human grey or white matter was a clear transparent colorless oily solid, soluble in hexane, cyclohexane, and chloroform
Summary
The solvents used were A.C.S. Reagent Grade and were redistilled prior to use. Klenk and Bohm [1] and Debuch [2] reported that these phospholipids isolated from brain contain a variety of fatty acids, which were studied by low temperature crystallization and alkali isomerization. In none of the subjects studied was there evidence of cerebral pathology on gross examination or after sectioning of the cerebral cortex. A 55 year old Caucasian male who had died suddenly from an acute myocardial infarction. A 77 year old Caucasian male who died of a myocardial infarction. The patient had a past history of mild diabetes mellitus.
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