Abstract
Glycerophosphatides were isolated from ox brain gray matter by column chromatography. The fatty acid compositions of ethanolamine glycerophosphatides (EGP), serine glycerophosphatides (SGP), and choline glycerophosphatides (CGP) were determined by gas-liquid chromatography. The positional distribution of fatty acids in these glycerophosphatides were determined by phospholipase A hydrolysis (Habu habu venom). C(20) and C(22) polyunsaturated acids were confined almost exclusively to the 2-position of these lipids, where they comprised the majority of 2-substituents in EGP and SGP (oleic acid predominated in this position in CGP). In the 1-position, palmitoyl was the major substituent in CGP, stearoyl in SGP, and stearoyl or the corresponding alk-1-enyl group in EGP.
Highlights
Glycerophosphatides were isolated from ox brain gray matter by column chromatography
This paper reports the positional distribution of fatty acids in glycerophosphatides from bovine cerebral gray matter
Thernajor fatty acids of ethanolamine glycerophosphatides (EGP) were 18:0, 18:1, 20 :4, and 22 :6, the last-named comprising somewhat more than 27% of the total fatty acids
Summary
Glycerophosphatides were isolated from ox brain gray matter by column chromatography. The fatty acid compositions of ethanolamine glycerophosphatides (EGP), serine glycerophosphatides (SGP), and choline glycerophosphatides (CGP) were determined by gas-liquid chromatography. The positional distribution of fatty acids in these glycerophosphatides were determined hy phospholipase A hydrolysis (Habu habu venom). CZOand CZ2polyunsaturated acids were confined almost exclusively to the 2-position of these lipids, where they comprised the majority of 2-substituents in EGP and SGP (oleic acid predominated in this position in CGP). Predominant species of phospholipid gray matter contain an extraordinarily high content of C Za~nd CZZpolyunsaturated fatty acids; 40-60% of the fatty acids of ethanolamine glycerophosphatides (EGP) and serine glycerophosphatides (SGP) from human brain gray matter are of this type [1]. This paper reports the positional distribution of fatty acids in glycerophosphatides from bovine cerebral gray matter
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