Quantification Analysis of 13 Organic Components and 8 Inorganic Elements in Angelica Sinensis Radix and Its Different Parts Combined with Chemical Recognition Pattern.

Xi Li,Yixin Yao,Shanshan Gao,Xiaoxiao Wang,Chang An,Fangtao Xiang,Yangli Dong

doi:10.1155/2020/8836184

Abstract

Angelica Sinensis Radix (Danggui, DG) is one of the most commonly prescribed traditional Chinese medicines. The organic components include phthalides and phenolic acids. Meanwhile, inorganic elements play an important role in clinical effect. DG and its different parts have different effects. There is no relevant report on the analysis of organic compounds and inorganic elements among them. Therefore, ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry was developed for the simultaneous determination of 13 organic components (8 phthalides and 5 phenolic acids), and 8 inorganic elements were determined by inductively coupled plasma mass spectrometry. The contents of 32 samples were analyzed by orthogonal partial least squares discrimination analysis, hierarchical cluster analysis, and least-significant difference of one-way analysis of variance. The results showed that the differences were significant among DG and its different parts. 11 difference markers (Ca, Z-ligustilide, Mg, Mn, Fe, Na, K, Cu, Zn, coniferyl ferulate, and senkyunolide A) were obtained by variable importance for the project. These difference markers were some different among DG and its different parts, especially Z‐ligustilide, coniferyl ferulate, Mg, Zn, the differences were significant. This study can provide a reference for DG research.

Highlights

Angelica Sinensis Radix (Danggui, DG), the dried root of Angelica sinensis (Oliv) Diels. (Umbelliferae), is one of the most commonly prescribed traditional Chinese medicines (TCM)
Modern studies have shown that DG can increase red blood cells and hemoglobin, improve hemorheology and acute myocardial infarction, etc [2,3,4]. e organic bioactive components mainly consist of phthalides and phenolic acids
In order to obtain an accurate and sensitive quantitative method by UHPLC-MS/ MS, individual solutions of all standard compounds were determined with the electrospray ionization (ESI) source by a full-scan mass spectrometry (MS) method and in both positive and negative modes to optimize the parameters of cone voltage (CV) and collision energy (CE) with the highest

Summary

Introduction

Angelica Sinensis Radix (Danggui, DG), the dried root of Angelica sinensis (Oliv) Diels. (Umbelliferae), is one of the most commonly prescribed traditional Chinese medicines (TCM). Modern research indicates that the efficacy of DG is strongly related with uterus disease and cardiovascular and cerebrovascular diseases [18] It indicates that these inorganic elements are the bioactive components of DG. Erefore, the UHPLC-MS/MS method was developed to simultaneously determine 13 organic components (chlorogenic acid, caffeic acid, vanillin, ferulic acid, senkyunolide I, senkyunolide H, coniferyl ferulate, Z-ligustilide, butylphthalide, senkyunolide A, butylidenephthalide, neocnidilide, and levistilide A) in different parts of DG.

Materials and Methods

Determination of Inorganic Elements by ICP-MS

Results and Discussion

Senkyunolide A

Validation of Methodology

10 Constituents Senkyunolide A Na Mg K Ca Mn Fe Cu Zn