Abstract
Three commercial kits for assaying alkaline phosphatase under optimized conditions were compared. The manufacturers stated that the optimized conditions conformed to the recommendations of the German Society for Clinical Chemistry. Measurement of pH, magnesium and p-nitrophenyl phosphate showed differences from the German recommended values, differences between kits and differences between vials within the same kit. These differences affected the results of alkaline phosphatase determinations on patients’ sera and lyophilized control materials, depending on the source and type of isoenzyme present. Three commercial kits for assaying alkaline phosphatase under optimized conditions were compared. The manufacturers stated that the optimized conditions conformed to the recommendations of the German Society for Clinical Chemistry. Measurement of pH, magnesium and p-nitrophenyl phosphate showed differences from the German recommended values, differences between kits and differences between vials within the same kit. These differences affected the results of alkaline phosphatase determinations on patients’ sera and lyophilized control materials, depending on the source and type of isoenzyme present.
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