Quality Control and Phytochemical Profiling of a Polyherbal Traditional Indian Medicine by GC-MS method

Abstract

Introduction: Gomutra (Cow's urine) has been described to be the most effective substance of animal origin. It is useful in treating various diseases due to its pharmacological activities. Nevertheless, due to its pungent smell, palatability, shelf life and fresh availability have always remained a problem. So it is very much essential to modify it into different dosage forms. One of its traditional dosage forms is Gomutrasava, an alcoholic fermented product indicated in the management of Shvitra (vitiligo). Aim & objective: The study is planned for Pharmaceutical preparation and quality control assessment of Gomutrasava. Material & Methods: Gomutrasava was prepared according to a reference from Astanga Hrudaya. This formulation was tested for organoleptic characters and physicochemical parameters, including Gas Chromatography-Mass Spectrometry (GC-MS). Observation & Results: After fermentation colour of Gomutrasava appeared dark brown with specific cow urine smell. pH, specific gravity at 25ºC, reftractive index, brix, viscocity, total solid content, alcohol content, reducing sugar, non reducing sugar of Gomutrasava were 5.41, 0.967 wt/ml, 1.394, 37º, 1.38/sec, 37%, 6%, 0.96%, 0.07% respectively and test for methanol and microbial contamination were negative. GC-MS revealed some compounds attributed to antimicrobial, anticancerous and antifungal activities. Conclusion Thus this data combined with analytical data will be used for clinical study and treatment of Shvitra (Vitiligo), Kushtha (Leprosy) and various skin diseases.