Determination of the chemical components, antioxidant and antifungal activities of essential oil and plant extract of Salvia candidissima Vahl.

Abstract

The aim of this study is to investigate the chemical components, antioxidant and antifungal activities of the essential oil and plant methanolic extract of Salvia candidissima Vahl. plant, which is one of the sage species. By using Gas Chromatography (GC) and Gas Chromatography Mass Spectrometry (GC-MS) method, it was determined that there are 54 components in the essential oil of S. candidissima. These identified components were found to constitute 99.98% of the essential oil. The main components of the essential oil were determined as Spathulenol (12.75%), Caryophyllene oxide (8.67%), Ledene oxide (6.98%) and o-Cymene (6.03%). A antifungal activity of the essential oil against Rhizoctonia solani and Aternaria solani was found. As a result of the study, it was determined that the mycelial growth of A. solani was inhibited by 57.92% as a result of application of 10 μL petri-1 essential oil, while that of R. solani was inhibited by 51.87%. As a result of the antioxidant study conducted with plant extract, the values of Free Radical Scavenging DPPH (lC50 22.96 ± 0.45), Iron Reduction Power (FRAP 1.20 ± 0.16 mmol TE g-1 extract), Copper Reduction Power (CUPRAC 3.30 ± 0.12 mmol TE g-1 extract) and Free Cation Radical Scavenging TEAC (lC50 9.25 ± 0.40 (µg ml-1)) were determined. The Total Phenolic (TP) and Total Flavonoid (TF) contents were found as 83.53 ± 5.92 mg GAE g-1 extract and 59.02 ± 3.59 mg QE g-1 extract, respectively. These results showed that the essential oil and plant methanolic extract of S.candidissima has a significant antifungal activity against plant pathogenic fungi and strong antioxidant activity.