Qualitative and quantitative estimation of gallic acid in flower galls of Crataeva religiosa through FT-IR and HPTLC techniques

Abstract

In the present study, FT- IR (FourierTransform Infrared spectroscopy) analysis of galled flower was conducted to qualitatively identify gallic acid while comparative HPTLC (High-Performance ThinLayer Chromatography) studies of normal and galled flower extracts confirmed the gallic acid quantitatively. The quantitative estimation of gallic acid was carried out on silica gel 60 F24HPTLC plates (stationary phase). The plates were developed in a solvent system as prepared with Toluene, Acetone and Glacial acetic acid at a ratio of 3:1:2, in a CAMAG glass twin trough chamber of 20X10 cm, at a temperature of 2 20C. Detection was carried out densitometrically at 220 nm. The FTIR analysis was done with a spectrophotometer system, which was used to identify and analyze the characteristic peaks and their respective functional groups. HPTLC analysis revealed that gallic acid content increased almost two folds in gall tissues as compared to normal tissues. C. religiosa flower galls can be utilized medicinally due to enhanced gallic acid.This is the first report of the occurrence of gallic acid in flower galls of C.religiosa.