Qualitative analysis of phytochemicals and anti-bacterial screening using ZnS/Mn nanoparticles encapsulated with ethanolic extract of Nyctanthes arbor-tristis Linn.

Abstract

Nyctanthes arbor-tristis Linn. (Nyctanthaceae) is one among the most useful traditional plants in India. It is a valuable medicinal plant used in Ayurveda. Various parts of the plant like fruits, leaves, seeds, flowers, barks and stem contain many phytochemicals and are used for the treatment and management of various diseases. Present investigation deals with the qualitative analysis of phytochemicals and antibacterial screening of dried leaf and flower extracts prepared in ethyl acetate, ethanol and benzene. The effect of ZnS/Mn nano particles coated with the ethanolic extract of leaf and flower was also reported. The qualitative analysis of the leaf and flower extracts revealed the presence of various classes of phytochemicals such as alkaloids, flavonoids, coumarins, phenols, resins, tanins, and steroids. The antibacterial potential of the extracts were evaluated on gram-positive (Bacillus subtilis) and gram-negative (Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas pyogens) bacteria in terms of zone of inhibition using agar well diffusion method.In the anti-bacterial screening, it is evident that flower has more anti-bacterial property than leaf. Among the three solvent extracts used, benzene gave no or poor zone of inhibition while ethyl acetate gave maximum zone of inhibition and the ethanolic extract gave only moderate zone of inhibition. ZnS/Mn nanoparticle encapsulated with ethanolic extracts of leaf and flower was screened for anti-bacterial activity against ethanolic extract. The ZnS/Mn encapsulated extracts gave more zone of inhibition than the extract alone. The results of this study indicates the potential of ZnS/Mn nanoparticles as a strong antibacterial agent which can be useful for antimicrobial applications. Further studies in this aspect has the potential to lead towards a few more astonishing facts towards the anti-biotic property of ZnS/Mn nanoparticles coated with the extracts.